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barcode adapter ligation  (New England Biolabs)


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    New England Biolabs barcode adapter ligation
    Barcode Adapter Ligation, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/barcode+ligation/Blunt%2FTA+Ligase+Master+Mix/pm41980963-509-22-27
    Average 99 stars, based on 1169 article reviews
    barcode adapter ligation - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Functional characterization of a novel plant growth-promoting rhizobacterium enhancing root growth and salt stress tolerance.
    Article Snippet: For sequencing, 4 μg DNA was used, and sequencing was conducted using both Illumina (San Diego, CA, USA) and Oxford Nanopore Technologies (ONT; Oxfordshire, UK) platforms. .. Bacterial strain sequencing and assembly Libraries for long-read sequencing were prepared with end repair, dA-tailing, barcode ligation, and adapter ligation using the NEBNext® UltraTM End Repair/dA-Tailing Module [Cat. No. E7546, New England Biolabs Co. (NEB), Ipswich, MA, USA], FFPE Repair Mix NEBNext Quick Ligation Module (Cat. No. E6056, NEB), and Native Barcoding Kit (Cat. No. SQK-NBD114.24, ONT). .. Ligated DNA was purified, and long-read sequencing was conducted using a MinION Mk1C device R10.4.1 (Cat. No. MIN-101 C, ONT) with a SpotON Flow Cell (Cat. No. FLO-MIN114, ONT), according to the manufacturer’s instructions and managed via MinKNOW software v4.2.5 (ONT).

    Article Title: Circulation dynamics of West Nile virus in Germany, 2023 and 2024.
    Article Snippet: .. For the subsequent 240 processing of amplicons for WGS using MinION technology (Oxford 241 Nanopore Technologies (ONT), Oxford Science Park, United 242 Kingdom) the most recent kits for barcode ligation (Native Barcoding 243 Kit 24 V14, REF: SQK-NBD114.24, ONT and NEBNext Quick Ligation 244 Module, Blunt/TA Ligase Master Mix, M 0367L, New England 245 Biolabs), library preparation (Ligation Sequencing Kit XL, SQK246 LSK1110-XL, ONT) and flow cell priming (Flow Cell Priming Kit, EXP- AR TIC LE IN PR ES S 247 FLP002, ONT) as well as a new generation of flow cells (MinION 248 GridION Flow Cell R10 Version, ONT) were utilized in comparison to 249 previously described protocols (23). ..

    Article Title: Genomic analysis and antimicrobial resistance of Vibrio cholerae isolated during Zambia’s 2023 cholera epidemic
    Article Snippet: Specifically, library preparation followed the Oxford Nanopore Technology (ONT) 1D Native Barcoding Genomic DNA protocol, with modifications [ ]. .. These included DNA repair using NEBNext Companion Module (New England Biolabs), barcode ligation using NEB Blunt/TA Ligase and EXP-NBD104 native barcode kit, pooling of 10–12 DNA samples (total 1 μg DNA) and adapter ligation using NEBNext Quick Ligation Module and SQK-LSK109 ligation sequencing kit. ..

    Article Title: Genomic analysis and antimicrobial resistance of Vibrio cholerae isolated during Zambia's 2023 cholera epidemic.
    Article Snippet: Specifically, library preparation followed the Oxford Nanopore Technology (ONT) 1D Native Barcoding Genomic DNA protocol, with modifications [22]. .. These included DNA repair using NEBNext Companion Module (New England Biolabs), barcode ligation using NEB Blunt/TA Ligase and EXP- NBD104 native barcode kit, pooling of 10–12 DNA samples (total 1 μg DNA) and adapter ligation using NEBNext Quick Ligation Module and SQK- LSK109 ligation sequencing kit. ..

    Article Title: Circulation of West Nile virus and Usutu virus in birds in Germany, 2021 and 2022.
    Article Snippet: This was followed by a purification step using AMPure XP SPRI reagent (Cat. No. A63881, Beckman Coulter, Inc., Indianapolis, USA), which is the means of purifying intermediate nucleic acid products throughout the entire protocol. .. The library preparation commenced with pre-treatment of amplicon strand ends for barcode ligation via NEBNext Ultra II End Repair/dA-Tailing Module (Cat. No. E7546, New England Biolabs (NEB), Ipswich, USA) followed by barcode ligation (NEBNext Ultra II Ligation Module, Cat. No. E7595, NEB and Native Barcoding Expansion Kit 1-12/13-24, Cat. No. EXP-NBD104/-114, ONT), motor protein ligation (NEBNext Quick Ligation Module, Cat. No. E6056, NEB) and the addition of loading beads (Ligation Sequencing Kit, Cat. No. SQK-LSK109, ONT). ..

    Article Title: Functional characterization of a novel plant growth-promoting rhizobacterium enhancing root growth and salt stress tolerance
    Article Snippet: For sequencing, 4 μg DNA was used, and sequencing was conducted using both Illumina (San Diego, CA, USA) and Oxford Nanopore Technologies (ONT; Oxfordshire, UK) platforms. .. Libraries for long-read sequencing were prepared with end repair, dA-tailing, barcode ligation, and adapter ligation using the NEBNext ® UltraTM End Repair/dA-Tailing Module [Cat. No. E7546, New England Biolabs Co. (NEB), Ipswich, MA, USA], FFPE Repair Mix NEBNext Quick Ligation Module (Cat. No. E6056, NEB), and Native Barcoding Kit (Cat. No. SQK-NBD114.24, ONT). .. Ligated DNA was purified, and long-read sequencing was conducted using a MinION Mk1C device R10.4.1 (Cat. No. MIN-101 C, ONT) with a SpotON Flow Cell (Cat. No. FLO-MIN114, ONT), according to the manufacturer’s instructions and managed via MinKNOW software v4.2.5 (ONT).

    Ligation:

    Article Title: Functional characterization of a novel plant growth-promoting rhizobacterium enhancing root growth and salt stress tolerance.
    Article Snippet: For sequencing, 4 μg DNA was used, and sequencing was conducted using both Illumina (San Diego, CA, USA) and Oxford Nanopore Technologies (ONT; Oxfordshire, UK) platforms. .. Bacterial strain sequencing and assembly Libraries for long-read sequencing were prepared with end repair, dA-tailing, barcode ligation, and adapter ligation using the NEBNext® UltraTM End Repair/dA-Tailing Module [Cat. No. E7546, New England Biolabs Co. (NEB), Ipswich, MA, USA], FFPE Repair Mix NEBNext Quick Ligation Module (Cat. No. E6056, NEB), and Native Barcoding Kit (Cat. No. SQK-NBD114.24, ONT). .. Ligated DNA was purified, and long-read sequencing was conducted using a MinION Mk1C device R10.4.1 (Cat. No. MIN-101 C, ONT) with a SpotON Flow Cell (Cat. No. FLO-MIN114, ONT), according to the manufacturer’s instructions and managed via MinKNOW software v4.2.5 (ONT).

    Article Title: Circulation dynamics of West Nile virus in Germany, 2023 and 2024.
    Article Snippet: .. For the subsequent 240 processing of amplicons for WGS using MinION technology (Oxford 241 Nanopore Technologies (ONT), Oxford Science Park, United 242 Kingdom) the most recent kits for barcode ligation (Native Barcoding 243 Kit 24 V14, REF: SQK-NBD114.24, ONT and NEBNext Quick Ligation 244 Module, Blunt/TA Ligase Master Mix, M 0367L, New England 245 Biolabs), library preparation (Ligation Sequencing Kit XL, SQK246 LSK1110-XL, ONT) and flow cell priming (Flow Cell Priming Kit, EXP- AR TIC LE IN PR ES S 247 FLP002, ONT) as well as a new generation of flow cells (MinION 248 GridION Flow Cell R10 Version, ONT) were utilized in comparison to 249 previously described protocols (23). ..

    Article Title: Genomic analysis and antimicrobial resistance of Vibrio cholerae isolated during Zambia’s 2023 cholera epidemic
    Article Snippet: Specifically, library preparation followed the Oxford Nanopore Technology (ONT) 1D Native Barcoding Genomic DNA protocol, with modifications [ ]. .. These included DNA repair using NEBNext Companion Module (New England Biolabs), barcode ligation using NEB Blunt/TA Ligase and EXP-NBD104 native barcode kit, pooling of 10–12 DNA samples (total 1 μg DNA) and adapter ligation using NEBNext Quick Ligation Module and SQK-LSK109 ligation sequencing kit. ..

    Article Title: Genomic analysis and antimicrobial resistance of Vibrio cholerae isolated during Zambia's 2023 cholera epidemic.
    Article Snippet: Specifically, library preparation followed the Oxford Nanopore Technology (ONT) 1D Native Barcoding Genomic DNA protocol, with modifications [22]. .. These included DNA repair using NEBNext Companion Module (New England Biolabs), barcode ligation using NEB Blunt/TA Ligase and EXP- NBD104 native barcode kit, pooling of 10–12 DNA samples (total 1 μg DNA) and adapter ligation using NEBNext Quick Ligation Module and SQK- LSK109 ligation sequencing kit. ..

    Article Title: Circulation of West Nile virus and Usutu virus in birds in Germany, 2021 and 2022.
    Article Snippet: This was followed by a purification step using AMPure XP SPRI reagent (Cat. No. A63881, Beckman Coulter, Inc., Indianapolis, USA), which is the means of purifying intermediate nucleic acid products throughout the entire protocol. .. The library preparation commenced with pre-treatment of amplicon strand ends for barcode ligation via NEBNext Ultra II End Repair/dA-Tailing Module (Cat. No. E7546, New England Biolabs (NEB), Ipswich, USA) followed by barcode ligation (NEBNext Ultra II Ligation Module, Cat. No. E7595, NEB and Native Barcoding Expansion Kit 1-12/13-24, Cat. No. EXP-NBD104/-114, ONT), motor protein ligation (NEBNext Quick Ligation Module, Cat. No. E6056, NEB) and the addition of loading beads (Ligation Sequencing Kit, Cat. No. SQK-LSK109, ONT). ..

    Article Title: Functional characterization of a novel plant growth-promoting rhizobacterium enhancing root growth and salt stress tolerance
    Article Snippet: For sequencing, 4 μg DNA was used, and sequencing was conducted using both Illumina (San Diego, CA, USA) and Oxford Nanopore Technologies (ONT; Oxfordshire, UK) platforms. .. Libraries for long-read sequencing were prepared with end repair, dA-tailing, barcode ligation, and adapter ligation using the NEBNext ® UltraTM End Repair/dA-Tailing Module [Cat. No. E7546, New England Biolabs Co. (NEB), Ipswich, MA, USA], FFPE Repair Mix NEBNext Quick Ligation Module (Cat. No. E6056, NEB), and Native Barcoding Kit (Cat. No. SQK-NBD114.24, ONT). .. Ligated DNA was purified, and long-read sequencing was conducted using a MinION Mk1C device R10.4.1 (Cat. No. MIN-101 C, ONT) with a SpotON Flow Cell (Cat. No. FLO-MIN114, ONT), according to the manufacturer’s instructions and managed via MinKNOW software v4.2.5 (ONT).

    Adapter Ligation:

    Article Title: Functional characterization of a novel plant growth-promoting rhizobacterium enhancing root growth and salt stress tolerance.
    Article Snippet: For sequencing, 4 μg DNA was used, and sequencing was conducted using both Illumina (San Diego, CA, USA) and Oxford Nanopore Technologies (ONT; Oxfordshire, UK) platforms. .. Bacterial strain sequencing and assembly Libraries for long-read sequencing were prepared with end repair, dA-tailing, barcode ligation, and adapter ligation using the NEBNext® UltraTM End Repair/dA-Tailing Module [Cat. No. E7546, New England Biolabs Co. (NEB), Ipswich, MA, USA], FFPE Repair Mix NEBNext Quick Ligation Module (Cat. No. E6056, NEB), and Native Barcoding Kit (Cat. No. SQK-NBD114.24, ONT). .. Ligated DNA was purified, and long-read sequencing was conducted using a MinION Mk1C device R10.4.1 (Cat. No. MIN-101 C, ONT) with a SpotON Flow Cell (Cat. No. FLO-MIN114, ONT), according to the manufacturer’s instructions and managed via MinKNOW software v4.2.5 (ONT).

    Article Title: Genomic analysis and antimicrobial resistance of Vibrio cholerae isolated during Zambia’s 2023 cholera epidemic
    Article Snippet: Specifically, library preparation followed the Oxford Nanopore Technology (ONT) 1D Native Barcoding Genomic DNA protocol, with modifications [ ]. .. These included DNA repair using NEBNext Companion Module (New England Biolabs), barcode ligation using NEB Blunt/TA Ligase and EXP-NBD104 native barcode kit, pooling of 10–12 DNA samples (total 1 μg DNA) and adapter ligation using NEBNext Quick Ligation Module and SQK-LSK109 ligation sequencing kit. ..

    Article Title: Genomic analysis and antimicrobial resistance of Vibrio cholerae isolated during Zambia's 2023 cholera epidemic.
    Article Snippet: Specifically, library preparation followed the Oxford Nanopore Technology (ONT) 1D Native Barcoding Genomic DNA protocol, with modifications [22]. .. These included DNA repair using NEBNext Companion Module (New England Biolabs), barcode ligation using NEB Blunt/TA Ligase and EXP- NBD104 native barcode kit, pooling of 10–12 DNA samples (total 1 μg DNA) and adapter ligation using NEBNext Quick Ligation Module and SQK- LSK109 ligation sequencing kit. ..

    Article Title: Functional characterization of a novel plant growth-promoting rhizobacterium enhancing root growth and salt stress tolerance
    Article Snippet: For sequencing, 4 μg DNA was used, and sequencing was conducted using both Illumina (San Diego, CA, USA) and Oxford Nanopore Technologies (ONT; Oxfordshire, UK) platforms. .. Libraries for long-read sequencing were prepared with end repair, dA-tailing, barcode ligation, and adapter ligation using the NEBNext ® UltraTM End Repair/dA-Tailing Module [Cat. No. E7546, New England Biolabs Co. (NEB), Ipswich, MA, USA], FFPE Repair Mix NEBNext Quick Ligation Module (Cat. No. E6056, NEB), and Native Barcoding Kit (Cat. No. SQK-NBD114.24, ONT). .. Ligated DNA was purified, and long-read sequencing was conducted using a MinION Mk1C device R10.4.1 (Cat. No. MIN-101 C, ONT) with a SpotON Flow Cell (Cat. No. FLO-MIN114, ONT), according to the manufacturer’s instructions and managed via MinKNOW software v4.2.5 (ONT).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Functional characterization of a novel plant growth-promoting rhizobacterium enhancing root growth and salt stress tolerance.
    Article Snippet: For sequencing, 4 μg DNA was used, and sequencing was conducted using both Illumina (San Diego, CA, USA) and Oxford Nanopore Technologies (ONT; Oxfordshire, UK) platforms. .. Bacterial strain sequencing and assembly Libraries for long-read sequencing were prepared with end repair, dA-tailing, barcode ligation, and adapter ligation using the NEBNext® UltraTM End Repair/dA-Tailing Module [Cat. No. E7546, New England Biolabs Co. (NEB), Ipswich, MA, USA], FFPE Repair Mix NEBNext Quick Ligation Module (Cat. No. E6056, NEB), and Native Barcoding Kit (Cat. No. SQK-NBD114.24, ONT). .. Ligated DNA was purified, and long-read sequencing was conducted using a MinION Mk1C device R10.4.1 (Cat. No. MIN-101 C, ONT) with a SpotON Flow Cell (Cat. No. FLO-MIN114, ONT), according to the manufacturer’s instructions and managed via MinKNOW software v4.2.5 (ONT).

    Article Title: Functional characterization of a novel plant growth-promoting rhizobacterium enhancing root growth and salt stress tolerance
    Article Snippet: For sequencing, 4 μg DNA was used, and sequencing was conducted using both Illumina (San Diego, CA, USA) and Oxford Nanopore Technologies (ONT; Oxfordshire, UK) platforms. .. Libraries for long-read sequencing were prepared with end repair, dA-tailing, barcode ligation, and adapter ligation using the NEBNext ® UltraTM End Repair/dA-Tailing Module [Cat. No. E7546, New England Biolabs Co. (NEB), Ipswich, MA, USA], FFPE Repair Mix NEBNext Quick Ligation Module (Cat. No. E6056, NEB), and Native Barcoding Kit (Cat. No. SQK-NBD114.24, ONT). .. Ligated DNA was purified, and long-read sequencing was conducted using a MinION Mk1C device R10.4.1 (Cat. No. MIN-101 C, ONT) with a SpotON Flow Cell (Cat. No. FLO-MIN114, ONT), according to the manufacturer’s instructions and managed via MinKNOW software v4.2.5 (ONT).

    Whole Complete genome sequencing:

    Article Title: Circulation dynamics of West Nile virus in Germany, 2023 and 2024.
    Article Snippet: .. For the subsequent 240 processing of amplicons for WGS using MinION technology (Oxford 241 Nanopore Technologies (ONT), Oxford Science Park, United 242 Kingdom) the most recent kits for barcode ligation (Native Barcoding 243 Kit 24 V14, REF: SQK-NBD114.24, ONT and NEBNext Quick Ligation 244 Module, Blunt/TA Ligase Master Mix, M 0367L, New England 245 Biolabs), library preparation (Ligation Sequencing Kit XL, SQK246 LSK1110-XL, ONT) and flow cell priming (Flow Cell Priming Kit, EXP- AR TIC LE IN PR ES S 247 FLP002, ONT) as well as a new generation of flow cells (MinION 248 GridION Flow Cell R10 Version, ONT) were utilized in comparison to 249 previously described protocols (23). ..

    Comparison:

    Article Title: Circulation dynamics of West Nile virus in Germany, 2023 and 2024.
    Article Snippet: .. For the subsequent 240 processing of amplicons for WGS using MinION technology (Oxford 241 Nanopore Technologies (ONT), Oxford Science Park, United 242 Kingdom) the most recent kits for barcode ligation (Native Barcoding 243 Kit 24 V14, REF: SQK-NBD114.24, ONT and NEBNext Quick Ligation 244 Module, Blunt/TA Ligase Master Mix, M 0367L, New England 245 Biolabs), library preparation (Ligation Sequencing Kit XL, SQK246 LSK1110-XL, ONT) and flow cell priming (Flow Cell Priming Kit, EXP- AR TIC LE IN PR ES S 247 FLP002, ONT) as well as a new generation of flow cells (MinION 248 GridION Flow Cell R10 Version, ONT) were utilized in comparison to 249 previously described protocols (23). ..

    Amplification:

    Article Title: Circulation of West Nile virus and Usutu virus in birds in Germany, 2021 and 2022.
    Article Snippet: This was followed by a purification step using AMPure XP SPRI reagent (Cat. No. A63881, Beckman Coulter, Inc., Indianapolis, USA), which is the means of purifying intermediate nucleic acid products throughout the entire protocol. .. The library preparation commenced with pre-treatment of amplicon strand ends for barcode ligation via NEBNext Ultra II End Repair/dA-Tailing Module (Cat. No. E7546, New England Biolabs (NEB), Ipswich, USA) followed by barcode ligation (NEBNext Ultra II Ligation Module, Cat. No. E7595, NEB and Native Barcoding Expansion Kit 1-12/13-24, Cat. No. EXP-NBD104/-114, ONT), motor protein ligation (NEBNext Quick Ligation Module, Cat. No. E6056, NEB) and the addition of loading beads (Ligation Sequencing Kit, Cat. No. SQK-LSK109, ONT). ..



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